N6-Methyladenine DNA Modification in Drosophila

N6-Methyladenine DNA Modification in Drosophila

DNA N(6)-methyladenine (6mA) modification is commonly found in microbial genomes and plays important functions in regulating numerous biological processes in bacteria. However, whether 6mA occurs and what its potential roles are in higher-eukaryote cells remain unknown. Here, we show that 6mA is present in Drosophila genome and that the 6mA modification is dynamic and is regulated by the Drosop...

N6-Methyladenine: A Conserved and Dynamic DNA Mark.

Chromatin, consisting of deoxyribonucleic acid (DNA) wrapped around histone proteins, facilitates DNA compaction and allows identical DNA codes to confer many different cellular phenotypes. This biological versatility is accomplished in large part by posttranslational modifications to histones and chemical modifications to DNA. These modifications direct the cellular machinery to expand or comp...

N-Methyladenine DNA Modification in Human Genome

Molecular analysis of N6-methyladenine patterns in Tetrahymena thermophila nuclear DNA.

We have cloned two DNA fragments containing 5'-GATC-3' sites at which the adenine is methylated in the macronucleus of the ciliate Tetrahymena thermophila. Using these cloned fragments as molecular probes, we analyzed the maintenance of methylation patterns at two partially and two uniformly methylated sites. Our results suggest that a semiconservative copying model for maintenance of methylati...

N6-methyladenine: a potential epigenetic mark in eukaryotes

Methylation modifications in DNA in the forms of 5-methylcytosine (5mC) and N 6-methyladenine (6mA) are one of the most important epigenetic marks that have been proposed to regulate gene expression and control numerous cellular and biological processes. The prevailing view is that, while 5mC serves as the predominant type, if not the only type, of methylated base in mammals to regulate gene ex...